SEM-FIB Aquilos2 ThermoFisher microscope

ThermoFisher's Aquilos2 microscope is a state-of-the-art high-resolution imaging SEM (source up to 30 kV) with a FIB column for milling (Ga ion source up to 30 kV). The system is equipped with a liquid nitrogen cryo-stage, which opens up the possibilities for biological sample analysis. The system can accommodate TEM grids and allows easy transfer during sample preparation, avoiding water contamination. The system also includes an in situ sputtering coating system. In addition, Aquilos2 is equipped with fluorescence correlative imaging for Scanning Electron-Light Microscopy. A micromanipulator is installed in the instrument for the extraction of lamellae suitable for TEM analysis.

Thanks to the cryo-stage, this microscope is suitable for biological samples, especially cells or tissues. Cells must be (at the moment) seeded and cultured in TEM grids to be inserted into the microscope. It is also possible to work with other heat-sensitive materials, such as polymers or lithium-based materials.

The laboratory is also equipped with a sputter coating system for carbon coating or plasma treatment. A biochemical preparation laboratory is also available. Complementary instruments and characterization tools are present as well (optical microscope, chemical fume hood, ultrapure water source).

Facility manager: Simone Dal Zilio (Facility Manager, Technologist).